Description

[APOE Gene Testing || Alzheimer’s Disease Prevention]

[ApoE Gene Polymorphism and Alzheimer’s Disease] The ApoE gene (Apolipoprotein E), located on chromosome 19, exhibits significant genetic polymorphism, with two common single nucleotide polymorphisms (rs429358 and rs7412). These polymorphisms are closely associated with Alzheimer’s disease. ApoE genetic polymorphism testing, based on extensive scientific research, enables early prevention of Alzheimer’s disease.

[ApoE Gene Structure] The ApoE gene is located on chromosome 19, spans 3.7Kb, and contains 4 exons. It has 2 SNP sites (rs429358 and rs7412) and 3 alleles (ε2, ε3, ε4), with ε3 being the most common mutation. These combine to form 6 major genotypes: ε2/ε2, ε2/ε3, ε2/ε4, ε3/ε3, ε3/ε4, ε4/ε4.

[ApoE Gene and Alzheimer’s Disease Risk] Although the three ApoE proteins differ by only one amino acid residue in primary structure, their spatial conformations vary significantly, resulting in distinctly different phenotypic effects in disease.

[Genotype Analysis Table]

Alzheimer’s disease (AD) is a common neurodegenerative disorder characterized by β-amyloid (Aβ) plaques and hyperphosphorylated protein neurofibrillary tangles. About 60-80% of AD patients have at least one E4 allele. Research shows that carrying one E4 allele increases risk 2-4 fold, while two E4 alleles increase risk 8-12 fold. E4 carriers develop symptoms approximately 12 years earlier than non-carriers.

The ApoE genotype influences lifetime health and lifestyle choices, guiding dietary habits and disease prevention strategies, and determining individualized treatment approaches.

[Detection Principle] Shanghai Biowing Applied Biotechnology Co., Ltd. employs dual-color fluorescence qPCR and melting curve analysis to simultaneously detect both SNP sites (rs429358 and rs7412). The combination of these results determines the APOE protein subtype and predicts individual Alzheimer’s disease risk.